Set up posters for gallery walk
1. Gallery walk
2. Review for final exam
Final exam tomorrow- finish final exam review
Warm-up 3: How many copies of DNA would you have after 7 rounds of PCR?
1. Lab: Paperclip PCR
2. Restriction enzyme practice
PCR lab questions
1.Describe the 3 main steps in PCR
2.What steps were used in OUR LAB to mimic the steps?
3.How were the steps we used in our lab different from real PCR (besides not using real DNA)?
4.What is the purpose of the primer in PCR?
5.Explain the importance of PCR as a discovery as it relates to genetic studies. Be thorough.
6.The person who discovered PCR won a Nobel Prize in chemistry. Do you think this process is worthy of a Nobel Prize? Why or why not?
7.Besides having more paperclips, how would you improve this lab so it is more like REAL PCR?
8.How many copies of DNA would you get after 4 rounds? 17 rounds? 20 rounds? 117 rounds?
HW: GMO food day tomorrow, bring your food. Answer the following questions in your notebook:
1. Does your food contain GMOs?
2. How do you know?
3. Which ingredients?
Warm-up 2: Identify these restriction enzymes as making either blunt or sticky ends
a) ATC-G b) AA-GG c) G-CATAG d) TTT-CCC
1. Correct restriction enzymes
2. Notes: Polymerase Chain Reaction
3. Practice: More restriction enzymes and PCR
4. Gallery walk if time permits
Warm-up 1: The DNA fingerprint below is from a crime scene. The blood stain is thought to be from the suspect. Who most likely committed the crime according to DNA evidence?
1. Correct DNA fingerprint assignment
2. Notes: Genetically modified organisms
3. Practice: Restriction enzymes